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Exome sequencing

Exome sequencing is a strategy to selectively sequence the coding regions of the genome. In the human genome, exons constitute about 1% of its genome and that the protein coding regions of the human genome constitute about 85% of the disease-causing mutations.MRDNA uses TruSeq exome enrichment kit (Illumina) for the sample library preparation. Sequence data generated from… Read more »

ChIP-Seq (Chromatin immunoprecipitation sequencing)

Chromatin immunoprecipitation (ChIP) is a powerful method for studying interactions between specific proteins and a genomic DNA region. MRDNA routinely performs ChIP-seq and provide cost effective high quality data, global binding site maps for a protein of interest and robust output. Please visit www.mrdnalab.com.               Our services – MR DNA Laboratory… Read more »

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NGS with longer read length and better quality at MR DNA

Next generation sequencing (NGS) has been instrumental in advancing scientific fields from human disease research to environmental and evolutionary science. MR DNA is your next generation sequencing and bioinformatics service provider and collaborator. We at MR DNA routinely perform next generation sequencing on Illumina platform. Recently Illumina has launched new reagent kits for the MiSeq… Read more »

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Cancer genomics at MR DNA

Cancer is a type of disease in which cells divide abnormally without control and are able to invade other tissues. One person dies from cancer each minute in the United States. As the population ages, this number is expected to increase. Thus, we need to understand cancer to control and ultimately conquer it. There are… Read more »

No featured image set for this post.

Metagenome Sequencing at MR DNA

Next generation sequencing (NGS) has been instrumental in advancing scientific fields from human disease research to environmental and evolutionary science. Genetic material recovered directly from environmental samples is termed as metagenomes. Metagenomics is defined as the direct genetic analysis of genomes contained with an environmental sample (Thomas et al., 2012). In other words, metagenomics refers… Read more »

No featured image set for this post.

Transcriptome sequencing

Biomedical research requires fast and accurate genome and transcriptome analysis for the investigation of cellular state, physiology, pathology and activity (Mutz et al., 2013). Transcriptome sequencing, also known as RNA-seq is profiling of mRNA in order to analyze and characterize the entire transcriptome and is applicable for both coding both coding mRNA and non-coding RNA…. Read more »

No featured image set for this post.

MicroRNA (miRNAs) detection using next generation sequencing (NGS) technologies

MicroRNAs are a class of short, non-coding, single-stranded RNAs (around 21–25 nucleotides in length) that act as post-transcriptional regulators in gene expression (Bartel 2009). It predominantly acts by binding to the 3′UTR of target mRNAs in the form of ribonucleoprotein complexes mediating mRNA destabilization and thereby translational repression (Krol et al., 2010). MicroRNAs are expressed… Read more »

No featured image set for this post.

Sequencing low diversity libraries at MR DNA

Next generation sequencing (NGS) has been instrumental in advancing scientific fields from human disease research to environmental and evolutionary science. It has been used to investigate sample diversity in amplicon libraries and has been instrumental in determining microbial diversity. We at MRDNA use Illumina MiSeq platform for 16S sequencing and analysis. With new version of… Read more »

No featured image set for this post.

Microsatellite identification at MR DNA

Microsatellites, or simple sequence repeats (SSRs) are regions of DNA that contain short tandem repeats (STRs) of 1 to 6 nucleotides. Microsatellites occur ubiquitously in all prokaryotic and eukaryotic genomes (Buschiazzo and Gemmell, 2006; Kelkar et al., 2008) and are popular markers for population genetics (Guichoux et al., 2011). Microsatellite markers are one of the… Read more »

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Genotyping

MRDNA does genotyping! Genotyping is the technique through which the variations in an organisms DNA are determined by comparing that organisms DNA to a reference sequence. Genoptyping of an organism also reveals its alleles, the various alternative forms of genes or groups of genes. It is also very important in the study of diseases, and… Read more »

  • Exome sequencing

    Exome sequencing is a strategy to selectively sequence the coding regions of the genome. In the human genome, exons constitute about 1% of its genome and that the protein coding regions of the human genome constitute about 85% of the disease-causing mutations.MRDNA uses TruSeq exome enrichment kit (Illumina) for the sample library preparation. Sequence data generated from… Read more »

    Read More
    Posted on February 5, 2015 by
  • truseq-chip09242013

    ChIP-Seq (Chromatin immunoprecipitation sequencing)

    Chromatin immunoprecipitation (ChIP) is a powerful method for studying interactions between specific proteins and a genomic DNA region. MRDNA routinely performs ChIP-seq and provide cost effective high quality data, global binding site maps for a protein of interest and robust output. Please visit www.mrdnalab.com.               Our services – MR DNA Laboratory… Read more »

    Read More
    Posted on January 27, 2015 by
  • NGS with longer read length and better quality at MR DNA

    Next generation sequencing (NGS) has been instrumental in advancing scientific fields from human disease research to environmental and evolutionary science. MR DNA is your next generation sequencing and bioinformatics service provider and collaborator. We at MR DNA routinely perform next generation sequencing on Illumina platform. Recently Illumina has launched new reagent kits for the MiSeq… Read more »

    Read More
    Posted on October 28, 2013 by
  • Cancer genomics at MR DNA

    Cancer is a type of disease in which cells divide abnormally without control and are able to invade other tissues. One person dies from cancer each minute in the United States. As the population ages, this number is expected to increase. Thus, we need to understand cancer to control and ultimately conquer it. There are… Read more »

    Read More
    Posted on October 25, 2013 by
  • Metagenome Sequencing at MR DNA

    Next generation sequencing (NGS) has been instrumental in advancing scientific fields from human disease research to environmental and evolutionary science. Genetic material recovered directly from environmental samples is termed as metagenomes. Metagenomics is defined as the direct genetic analysis of genomes contained with an environmental sample (Thomas et al., 2012). In other words, metagenomics refers… Read more »

    Read More
    Posted on October 25, 2013 by
  • Transcriptome sequencing

    Biomedical research requires fast and accurate genome and transcriptome analysis for the investigation of cellular state, physiology, pathology and activity (Mutz et al., 2013). Transcriptome sequencing, also known as RNA-seq is profiling of mRNA in order to analyze and characterize the entire transcriptome and is applicable for both coding both coding mRNA and non-coding RNA…. Read more »

    Read More
    Posted on October 25, 2013 by
  • MicroRNA (miRNAs) detection using next generation sequencing (NGS) technologies

    MicroRNAs are a class of short, non-coding, single-stranded RNAs (around 21–25 nucleotides in length) that act as post-transcriptional regulators in gene expression (Bartel 2009). It predominantly acts by binding to the 3′UTR of target mRNAs in the form of ribonucleoprotein complexes mediating mRNA destabilization and thereby translational repression (Krol et al., 2010). MicroRNAs are expressed… Read more »

    Read More
    Posted on October 25, 2013 by
  • Sequencing low diversity libraries at MR DNA

    Next generation sequencing (NGS) has been instrumental in advancing scientific fields from human disease research to environmental and evolutionary science. It has been used to investigate sample diversity in amplicon libraries and has been instrumental in determining microbial diversity. We at MRDNA use Illumina MiSeq platform for 16S sequencing and analysis. With new version of… Read more »

    Read More
    Posted on October 25, 2013 by
  • Microsatellite identification at MR DNA

    Microsatellites, or simple sequence repeats (SSRs) are regions of DNA that contain short tandem repeats (STRs) of 1 to 6 nucleotides. Microsatellites occur ubiquitously in all prokaryotic and eukaryotic genomes (Buschiazzo and Gemmell, 2006; Kelkar et al., 2008) and are popular markers for population genetics (Guichoux et al., 2011). Microsatellite markers are one of the… Read more »

    Read More
    Posted on October 25, 2013 by
  • Genotyping

    MRDNA does genotyping! Genotyping is the technique through which the variations in an organisms DNA are determined by comparing that organisms DNA to a reference sequence. Genoptyping of an organism also reveals its alleles, the various alternative forms of genes or groups of genes. It is also very important in the study of diseases, and… Read more »

    Read More
    Posted on July 22, 2013 by